Affinity chromatography of trypsin inhibitors on trypsinogen-agarose exchangers
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چکیده
منابع مشابه
Purification of hydrogenases by affinity chromatography on Procion Red-agarose.
The agarose-coupled triazine dye Procion Red HE-3B has been demonstrated to be applicable as an affinity gel for the purification of five diverse hydrogenases, namely the soluble, NAD-specific and the membrane-bound hydrogenase of Alcaligenes eutrophus, the membrane-bound hydrogenase of the N2-fixing Alcaligenes latus, the reversible H2-evolving and the unidirectional H2-oxidizing hydrogenase o...
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Uniform-sized agarose beads were prepared by emulsification technique in this study. Agarose (4% w/v) was dissolved in boiling water containing 0.9% sodium chloride and used as water phase. A mixture of liquid paraffin containing 3 wt% of tween 80 emulsifier was heated to 70-80 o C and used as oil phase. Then the heated oil phase was poured into the water phase and the mixture was stirred with ...
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Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. On the basis of previous estimates of the sugar content of the hormone, six lectins (wheat germ agglutinin, phytohemagglutinin, Ricinus communis 120, soybean agglutinin, concanavalin A, and limulin) were chosen for study. Only wheat ger...
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A simple and effective purification method for the src kinase, the transforming gene product of Rous sarcoma virus, has been developed by using affinity chromatography on casein-agarose and tyrosine-agarose columns. NaDodSO4/polyacrylamide gel electrophoresis and silver staining analysis showed that the purified kinase preparation was composed of a predominant polypeptide of 60,000-Da. In most ...
متن کاملCrystalline Chymo-trypsin and Chymo-trypsinogen
A new crystalline protein, chymo-trypsinogen, has been isolated from acid extracts of fresh cattle pancreas. This protein is not an enzyme but is transformed by minute amounts of trypsin into an active proteolytic enzyme called chymo-trypsin. The chymo-trypsin has also been obtained in crystalline form. The chymo-trypsinogen cannot be activated by enterokinase, pepsin, inactive trypsin, or calc...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1971
ISSN: 0014-5793
DOI: 10.1016/0014-5793(71)80140-0